Influence of bioaugmented fungi on tolerance, growth and phytoremediation ability of Prosopis juliflora Sw. DC in heavy metal-polluted landfill soil.

The research aimed to determine the influence of endophytic fungi on tolerance, growth and phytoremediation ability of Prosopis juliflora in heavy metal-polluted landfill soil. A consortium of 13 fungal isolates as well as Prosopis juliflora Sw. DC was used to decontaminate heavy metal-polluted landfill soil. Enhanced plant growth (biomass and root and shoot lengths) and production of carotenoids, chlorophyll and amino acids L-phenylalanine and L-leucine that are known to enhance growth were found in the treated P. juliflora. Better accumulations of heavy metals were observed in fungi-treated P. juliflora over the untreated one. An upregulated activity of peroxidase, catalase and ascorbate peroxidase was recorded in fungi-treated P. juliflora. Additionally, other metabolites, such as glutathione, 3,5,7,2',5'-pentahydroxyflavone, 5,2'-dihydroxyflavone and 5,7,2',3'-tetrahydroxyflavone, and small peptides, which include Lys Gln Ile, Ser Arg Ala, Asp Arg Gly, Arg Ser Ser, His His Arg, Arg Thr Glu, Thr Arg Asp and Ser Pro Arg, were also detected. These provide defence supports to P. juliflora against toxic metals. Inoculating the plant with the fungi improved its growth, metal accumulation as well as tolerance against heavy metal toxicity. Such a combination can be used as an effective strategy for the bioremediation of metal-polluted soil.

Plant-soil feedback and plant invasion: effect of soil conditioning on native and invasive Prosopis species using the plant functional trait approach.

Introduction: Invasive species have been identified as a major threat to native biodiversity and ecosystem functioning worldwide due to their superiority in spread and growth. Such superiority is explained by the invasional meltdown phenomena, which suggests that invasive species facilitate the establishment of more invasive species rather than native species by modifying the plant-soil feedback (PSF). Methods: We conducted a two-phase plant-soil feedback experiment using the native Prosopis cineraria and the invasive Prosopis juliflora in Oman. Firstly, we conditioned the soil by planting seedlings of native species, invasive species, native and invasive species "mixed", and unconditioned soil served as a control. Secondly, we tested the feedback of these four conditioned soil on the two species separately by measuring the productivity (total biomass) and the performance in the form of plant functional traits (plant height, specific leaf area (SLA), leaf nitrogen content (Nmass), leaf carbon content (Cmass) and specific root length (SRL) of native and invasive species as well as the nutrient availability in soil (soil organic carbon (SOC) and soil total nitrogen (STN)). Results and discussion: We found that the native species produced more biomass, best performance, and higher SOC and STN when grown in soil conditioned by native species, additionally, it gave lower biomass, reduced performance, and lower SOC and STN when grown in the soil conditioned by invasive and mixed species. These results suggest negative PSF for native species and positive PSF for invasive species in the soil conditioned by invasive species, which can be considered as red flag concerning the restoration of P. cineraria as an important native species in Oman, as such positive PSF of the invasive species P. juliflora will inhibit the regeneration of P. cineraria.

Prosopis juliflora (Sw.) DC.induces apoptotic-like programmed cell death in Leishmania donovani via over production of oxidative stress, mitochondrial dysfunction and ATP depletion.

Background: Leishmaniasis is endemic in more than 60 countries with a large number of mortality cases. The current chemotherapy approaches employed for managing the leishmaniasis is associated with severe side effects. Therefore there is a need to develop effective, safe, and cost affordable antileishmanial drug candidates. Purpose of the study: This study was designed to evaluate the in vitro antileishmanial activity of a Prosopis juliflora leaves extract (PJLME) towards the Leishmania donovani parasites. Material and methods: PJLME was evaluated for its cytotoxicity against the L. donovani parasites and the mouse macrophage cells. Further, various in vitro experiments like ROS assay, mitochondrial membrane potential assay, annexin v assay, cell cycle assay, and caspase 3/7 assay were performed to understand the mechanism of cell death. Phytochemical profiling of P. juliflorawas performed by utilizing HPTLC and GC-MS analysis. Results: PJLME demonstrated antileishmanial activity at a remarkably lower concentration of IC50 6.5 µg/mL. Of note, interestingly PJLME IC50 concentration has not demonstrated cytotoxicity against the mouse macrophage cell line. Performed experiments confirmed ROS inducing potential of PJLME which adversely affected the mitochondrial membrane potential and caused loss of mitochondrial membrane potential and thereby ATP levels. PJLME also arrested the cell cycle and induced apoptotic-like cell death in PJLME treated L. donovani promastigotes. Conclusion: The results clearly established the significance of Prosopis juliflora as an effective and safe natural resource for managing visceral leishmaniasis. The findings can be used as a baseline reference for developing novel leads/formulations for effective management of visceral leishmaniasis.

Enhancement of biosorption capability of imidazolium-based ionic liquid-treated Prosopis juliflora for the removal of malachite green from wastewater.

Due to its toxicity effect, treating toxic pollutants discharged from textile effluent is challenging for living beings. In the present study, the comparative biosorption potential of imidazolium-based ionic liquid-treated Prosopis juliflora (ILPJS) and untreated P. juliflora (PJS) was investigated for the removal of toxic pollutant, malachite green (MG) from aqueous solution. The textural, surface morphology, and functional analysis of ILPJS and PJS were examined using BET (Brunauer-Emmett-Teller) analysis, SEM (Scanning electron microscopy) analysis, and FTIR (Fourier-transform infrared spectroscopy) analysis. Textural property (BET surface area) and surface morphology containing irregular heterogeneous surface for ILPJS were significantly improved than PJS, thereby facilitating significant biosorption of MG. Based on the conventional optimization studies, the essential biosorption parameters for the removal of MG using ILPJS were found to be: initial pH (9.0), contact time (30 min), and biosorbent dosage (0.2 g). The maximum biosorption capacity of PJS and ILPJS were obtained to be 6.91 and 13.64 mg/g at 40 °C, respectively. The spontaneous and endothermic biosorption of MG was confirmed by thermodynamic analysis. The regeneration study indicated the greater reusability of ILPJS and PJS for MG removal till the fifth cycle. Based on the previous literature, this is the first report comparing the removal of toxic pollutant MG using ILPJS and PJS.

Prosopis juliflora is an invasive weed that causes a severe challenge to ecological diversity and rural livelihoods due to the continuous consumption of water throughout the year, leading to the depletion of groundwater reserves. To control its invasion and growth, weed has been applied as biosorbents to remove toxic pollutant, malachite green (MG). This is the first report comparing the pretreatment of P. juliflora using imidazolium-based ionic liquid (ILPJS) with raw P. juliflora (PJS) for the biosorption of MG. The biosorption capacity of ILPJS for MG removal was 1.97 times higher than PJS. The enhancement in biosorption capacity might be the possibility of better textural and surface morphology of chemically treated P. juliflora. Thermodynamic studies revealed the endothermic and spontaneous nature of the biosorption of MG on PJS. With the invasion of this weed over thousands of hectares of land in India, PJS is the ideal biosorbent for removing toxic chemical pollutants and preserving the groundwater level.

Production, optimization, and characterization of Ethiopian variant Prosopis juliflora based biodiesel.

Considering the need for biodiesel production from non-edible oil sources and taking into account the fact that Prosopis Juliflora (JF) is identified as a highly invasive species in Ethiopia, this research focuses on biodiesel production from a possible and promising alternative feedstock. The objective of this study is to analyze Ethiopian variant Juliflora based biodiesel (JFB) production through transesterification, carry out optimization by exploring the effects of various process parameters and characterization of functional groups (with GC-MS,FT-IR and NMR) including rheological behavior, not yet been reported earlier. As per ASTM protocol testing, the methyl ester of Juliflora has been found to have the following main fuel properties: kinematic viscosity (mm2/s) 3.395, cetane number 52.9, acid number (mgkoh/g) 0.28, density (gm/ml) 0.880, calorific value (MJ/kg) 44.4, methyl ester content (%) 99.8, and flashpoint (°C) 128, copper strip corrosion value 1a,%FFA (free fatty acid) 0.14. When compared with those of diesel, the viscosity, density, and flash point of JFB are seen to be higher than those of diesel, although it has a similar calorific value but more importantly higher than most of the other biodiesels. Based on an assessment using response surface methodology, methanol concentration together with catalyst loading, temperature, and reaction time are determined to be the most important influencing process parameters. The best molar ratio for methanolysis was observed to be 6:1 with a catalyst concentration of 0.5 wt% at 55 °C for 60 min for biodiesel yield at 65%. The JFB maximum yield of 130 ml at 70 min and the minimum yield of 40 ml at 10 min demonstrate that as mixing time increases, JFB yield tend to increase up to a certain time limit. The maximum raw oil yield rom crushed seed with hexane solvent was observed to be 480 ml within 3 days from 2.5 kg of crushed seed. The Fourier transform infrared analysis (FT-IR) revealed the presence of all desired functional groups necessary for biodiesel on OH radicals at wave numbers of 3314.40 cm-1, Aliphatic methyl C-H at 2942.48 cm- 1 with a functional group (CH-3-,-CH2-), and methylene C-H at 2832.59 cm-1. The gas chromatography-mass spectrometer (GC-MS) study confirmed the higher ester content present in the JFB with a higher unsaturation level of 68.81%. The fatty acid, oleic acid has a lower saturation level of 4.5%, while palmitic acid has a lower threshold level of 2.08%. The Rheometer test showed that shear stress and viscosity reduced with increasing temperature within the range of biodiesel requirements, and the Newtonian behavior was confirmed. The JFB has a fairly high viscosity and shear rate at low temperatures. The 1H NMR (nuclear magnetic resonance) study established that JFB has a necessary ingredient; and aliphatic resonances occur in the chemical shift region of 1.5-3.0 ppm. Significant regions indicate protons bound to heteroaromatics, aldehydes, as shown by 13C NMR spectrum. The findings from the FT-IR, GC-MS, 1H NMR, and 13C NMR are in agreement thus validating the presence of numerous functional groups in JFB as such. Since JFB possesses the requisite biodiesel fuel attributes, Prosopis Juliflora need to be pursued as a promising biodiesel feedstock in Ethiopia for alleviating the burden of imported fuels while also addressing difficulties with emissions released by the combustion of fossil fuels.

Prosopis juliflora (Sw.) DC phytochemicals induce apoptosis and inhibit cell proliferation signaling pathways, EMT, migration, invasion, angiogenesis and stem cell markers in melanoma cell lines.

ETHNOPHARMACOLOGICAL RELEVANCE: Prosopis juliflora (Sw.), DC is a xerophytic plant species that extensively grow in Asia, Africa, Australia, and Brazil. From ancient time P. juliflora is being utilized in various folk remedies for example in wound healing, fever, inflammation, measles, excrescences, diarrhea and dysentery. Traditionally, gum, paste, and smoke obtained from the leaves and pods are applied for anticancer, antidiabetic, anti-inflammatory, and antimicrobial purposes. AIM OF THE STUDY: Our previous studies have demonstrated the promising potential of Prosopis Juliflora leaves methanol extract (PJLME) against breast cancer, and suggested its possible integration as a complementary medicine for the effective management of breast cancer. However, evidence against how PJLME mechanistically target the cancer proliferative pathways and other targets is poorly understood. The basic aim of the present study was to understand the anti-melanoma potential of PJLME against B16f10 cells with possible mechanisms of action. MATERIALS AND METHODS: MTT assay was used to determine cell viability. Wound and transwell migration assay was performed to check migration potential of cells after PJLME treatment, while clonogenic assay was carried out to understand its colony inhibition actvity. Flow cytometry was used to perform annexin V/PI assay (apoptosis assay), ROS assay, cell cycle analysis. In-vitro angiogenesis assay was performed to check formation of capillary like vascular structure after PJLME treatment. Apoptotic genes, signaling pathways markers, EMT markers and stem cell markers were determined by western blotting. In-vivo BALB/C mice xenograft model study was performed to check the effect of PJLME on in-vivo melanoma tumor growth. RESULTS: The experimental outcome of the present study has clearly demonstrated the inhibition of growth, migration, invasion, colony formation and apoptosis inducing potential of PJLME against mouse melanoma cancer cells. Treatment of B16F10 melanoma cells with PJLME resulted in arrest of cell cycle at G0/G1 phase. Annexin V-FITC/PI assay confirmed the apoptosis inducing potential of PJLME in B16F10 and A375 melanoma cells. Furthermore, Western blot experiments confirmed that the treatment of PJLME downregulates the expression of anti-apoptotic gene like Bcl2 and increase the expression profile of pro-apoptotic genes like Bax, Bad, and Bak in B16F10 melanoma cells. HUVEC (Human umbilical vein endothelial cells) tube formation assay clearly demonstrated the anti-angiogenic potential of PJLME. The study also revealed that PJLME has potential to inhibit the Akt and Erk signaling pathways which are participating in cancer cell proliferation, migration, invasion etc. The outcome of qRT-PCR and immunoblotting analysis clearly unveiled that PJLME treatment leads to downregulation of epithelial-mesenchymal transition (EMT) as well as stem cell markers. Finally, the in-vivo animal xenograft model study also revealed the anti-melanoma potential of PJLME by significantly inhibiting the B16F10 melanoma tumor growth in BALB/c mice model. The LC-ESI-MS/MS analysis of PJLME showed the presence of variety of bioactive molecules associated with anticancer effects. CONCLUSION: The outcome of the present investigation clearly demonstrated the anti-melanoma potential of PJLME against B16f10 melanoma cells. PJLME can be explored as an adjuvant or complementary therapy against melanoma cancer, however further studies are required to understand the clinical efficacy of PJLME. Nevertheless, it can be further explored as a promising resource for identification of novel anticancer candidate drug.

Four Invasive Plant Species in Southwest Saudi Arabia Have Variable Effects on Soil Dynamics.

Predicting the direction and magnitude of change in soil dynamics caused by invasive plant species has proven to be difficult because these changes are often reported to be species- and habitat-specific. This study was conducted to determine changes in three soil properties, eight soil ions, and seven soil microelements under established stands of four invasive plants, Prosopis juliflora, Ipomoea carnea, Leucaena leucocephala, and Opuntia ficus-indica. Soil properties, ions, and microelements were measured in sites invaded by these four species in southwest Saudi Arabia, and these values were compared to the results for the same 18 parameters from adjacent sites with native vegetation. Because this study was conducted in an arid ecosystem, we predict that these four invasive plants will significantly alter the soil properties, ions, and microelements in the areas they invaded. While the soils of sites with the four invasive plant species generally had higher values for soil properties and ions compared to sites with native vegetation, in most instances these differences were not statistically significant. However, the soils within sites invaded by I. carnea, L. leucocephala, and P. juliflora had statistically significant differences for some soil parameters. For sites invaded by O. puntia ficus-indica, no soil properties, ions, or microelements were significantly different compared to adjacent sites with native vegetation. Sites invaded by the four plant species generally exhibited differences in the 11 soil properties, but in no instance were these differences statistically significant. All three soil properties and one soil ion (Ca) were significantly different across the four stands of native vegetation. For the seven soil microelements, significantly different values were detected for Co and Ni, but only among stands of the four invasive plant species. These results indicate that the four invasive plant species altered soil properties, ions, and microelements, but for most of the parameters we assessed, not significantly. Our results do not support our initial prediction, but are in general agreement with previous published findings, which indicate that the effects of invasive plants on soil dynamics vary idiosyncratically among invasive species and among invaded habitats.

Effects of prosopis juliflora on plant diversity on rangeland in Shilabo District, Somali Regional State, Ethiopia.

Shilabo District of Somali Regional State in Ethiopia is economically important for livestock production. The indigenous pasturelands are increasingly being invaded by Prosopis juliflora, thereby threatening their livestock production capacity. However, the ecological impact of Prosopis' invasion has yet to be investigated. This study was conducted to examine the effects of Prosopis juliflora on plant species abundance, diversity, and occurrence. Vegetation samples were collected from three 20-m by 20-m sub-plots nested within 100-m by 100-m main plots that were purposefully selected in target study sites and replicated three times in both invaded and uninvaded areas. The study recorded 44 plant species, of which thirteen species occurred in both invaded and uninvaded areas, 13 species in invaded areas and 24 in uninvaded areas. The collected data were analyzed using the Statistical Package for Social Sciences (SPSS) version 26, and an independent t-test was used to determine the statistical significance difference between invaded and uninvaded areas in terms of number, density, important value index, diversity, abundance, evenness, and richness of plant species. These plant parameters were recorded in invaded and uninvaded areas with species numbers of 678.33 and 1763, densities of 226.00 and 366.98/ha, abundances of 254.04 and 409.45, important value indexes of 15.00 and 9.68, Shannon diversity of 1.56 and 3.40, evenness of 0.52, and 0.99, and richness of 1.30 and 1.35, respectively. The uninvaded area had a significantly higher species diversity index (3.40) than the invaded area (1.56; P 0.00). Similarly, the number of plant species, density, abundance, important value index, species evenness, and richness were significantly higher in uninvaded areas than invaded areas (p < 0.000).

Can phytogenic additives replace monensin sodium in beef cattle feeding?

Monensin sodium is one of the most common ionophores used in livestock feeding; however, ionophores are condemned by organized consumer groups. Bioactive compounds from plants found in the seasonally dry tropical forest have similar mechanisms of action as ionophores. The aim was to investigate the effects of replacing monensin sodium with phytogenic additives on the nutritional efficiency of beef cattle. Five 14-month-old Nellore bulls (average body weight 452.68 ± 42.60 kg) were used in the study. The experiment was designed as a 5 × 5 Latin Square (five treatments and five 22-day experimental periods). Within each period, 15 days were used for adaptation of animals to experimental conditions and 7 days for data collection. Bulls were fed a control diet (without additives), monensin (a diet containing monensin sodium 40% as a synthetic additive), and three diets containing phytogenic additives prepared from Anadenanthera macrocarpa (Benth) Brenan, Mimosa tenuiflora (Willd) Poiret, or Prosopis juliflora (Sw.) DC. Nutritional efficiency was assessed through feed intake, nutrient digestibility, feeding behavior, and hematological parameters. Monensin and phytogenic additives did not influence (P > 0.05) feeding behavior or hematological parameters, but the nutrient intake was highest for bulls supplemented phytogenic additives (P < 0.05). Monensin supplementation did not influence (P > 0.05) feed intake. The phytogenic additives and monensin sodium increased (P < 0.05) the nutrient digestibility. Therefore, the phytogenic additives from P. juliflora, A. macrocarpa, and M. tenuiflora can be recommended to enhance the nutritional efficiency of confined Nellore cattle.

Thermal decomposition kinetics of Prosopis juliflora charcoal briquette using thermogravimetric analysis.

In the current study, the energy potential of Prosopis juliflora charcoal briquette sample was assessed using thermogravimetric analysis at heating rates 10 °C/min, 15 °C/min, and 20 °C/min under nitrogen atmosphere. The thermogravimetric study showed that the thermal devolatilization of the briquette sample occurred in four principal stages. The major degradation of the sample occurred in the fourth stage indicating that the significant mass loss occurred due to the fixed carbon that was abundant in the briquette sample. The activation energy was determined by employing five different model-free methods. The average activation energy attained for the briquette sample by Kissinger-Akahira-Sunose method, Flynn-Wall-Ozawa method, Tang method, Starink method, and Friedman method was 83.55 kJ/mol, 91.60 kJ/mol, 79.91 kJ/mol, 80.06 kJ/mol, and 96.74 kJ/mol, respectively. The frequency factor obtained in the study ranged between 1.42 × 103 and 6.23 × 107 min-1. The contracting sphere model was found to be closely related to the reaction model obtained for charcoal briquettes. The lower activation energy and frequency factor indicated rapid thermal degradation of the charcoal briquettes. The estimated thermodynamic parameters indicated that the thermal degradation process was endothermic in nature.

Competitive and cooperative adsorption analysis for dye removal from multicomponent system using Prosopis juliflora activated carbon.

In this study, performance evaluation of two adsorbents synthesized using invasive weed, i.e., Prosopis juliflora, was chemically activated using hydrochloric acid (HPJ) and sodium hydroxide (NPJ). The synthesized adsorbents HPJ and NPJ were subjected to SEM, EDX, XRD, FTIR, and porosimetry analysis for characterization and applied for adsorptive removal of rhodamine B (RB) and methyl orange (MO) dyes from monocomponent (MO/RB) and multicomponent (MO + RB) systems in batch mode. Meanwhile, the effect of operational parameters such as contact time, HPJ and NPJ dosage, MO/RB concentration, and [Formula: see text] on sorption of MO/RB dyes was investigated. The adsorption data was modeled through various kinetic and equilibrium models. On the other hand, the multi-dye sorption system was modeled using Langmuir competitive isotherm. Furthermore, the effect of presence of one dye on sorption of other and vice versa, i.e., competitive (antagonistic) and cooperative (synergistic) nature of sorption process, was investigated. From the results, it was observed that pseudo-second-order kinetic and Langmuir isotherm models best fit the adsorption kinetic and equilibrium data for sorption of MO and RB dyes using both HPJ and NPJ as adsorbents. Langmuir's maximum sorption ability (qm) of HPJ for sorption of MO and RB dyes was observed to be 12.77 mg/g and 9.95 mg/g, respectively, from the monocomponent system. On the other hand, qm of NPJ for sorption of MO and RB dyes was observed to be 10.51 mg/g and 8.69 mg/g, respectively. Langmuir's sorption ability (qm) was slightly higher in the MO + RB mixture in contrast to MO/RB. As a result, the sorption of MO/RB dyes from the MO + RB system showed synergistic nature. In conclusion, the HPJ and NPJ could be effectively used as sorbents for sorption of dyes from effluents.

Silvicultural Practices for Diversity Conservation and Invasive Species Suppression in Forest Ecosystems of the Bundala National Park, Sri Lanka.

Forest ecosystems in Sri Lanka are under pressure from intensive human activity and climate change. Invasive species are one of the greatest threats to autochthonous species and ecosystems. In Bundala National Park of Sri Lanka, there are efforts to control and limit the spreading of unwanted invasive Prosopis juliflora (Sw.) DC. and Opuntia dillenii (Ker-Gawl.) Haw., which poses a significant risk to natural ecosystem conservation. Nine different treatment variants (four replications) were used to test which management approach provides the control of Prosopis juliflora. This research is based on nine repeated measurements from 2017 to 2021 on 36 permanent research plots (each 625 m2) with 27 observed plant species and a total of 90,651 recorded plant individuals. The results confirmed that the dynamics of species richness, heterogeneity, and evenness showed significant differences between treatments during the five years of dynamics. The lowest species diversity was found in the control variant, followed by treatments based on the hard pruning and thinning of Prosopis juliflora trees. In contrast, strategies emphasizing the complete uprooting of Prosopis juliflora trees, replanting, and support of the natural regeneration of native species showed high species diversity and a high overall number of plant species. Generally, treatments had a significant effect on species diversity and the number of individuals of Prosopis juliflora, while changes in the overall number of plant species were more affected by time and succession. Silvicultural treatments including pruning, uprooting, and thinning have proven to be essential tools for nature conservation across various sites, aimed at enhancing habitat diversity in the face of ongoing climate change.

Dynamics of land use/land cover: implications on environmental resources and human livelihoods in the Middle Awash Valley of Ethiopia.

Quantifying the recent LULC changes and associated impacts on pastoral and agro-pastoral livelihood systems is important since the effects of LULC changes on environmental resources and human livelihood are not fully understood in our study area. This paper analysed the trend of land use/land cover (LULC) dynamics and its implications on natural resources and human livelihood in the Middle Awash Valley, Central Ethiopian Rift Valley. For the purpose, Landsat imageries of thematic mapper (1987), enhanced thematic mapper (2002) and operational land imager and thermal infrared sensor (2016) were employed and analysed using Remote Sensing and Geographic Information System (GIS) software and techniques, and qualitative data analysis had been performed as well. The results showed that cultivated land expanded at a rate of 2.6% year-1, whereas forestland and grassland shrunk at a rate of 1.2% year-1 and 2.4% year-1, respectively. The invasive Prosopis juliflora has been expanded from 3.7% in 1987 to 37.9% in 2016 at a rate of 1.2% year-1. The introduction of both small- and large-scale commercial irrigation farming and the implementation of villagization programme focused on transforming pastoralists into sedentary lifestyles. Consequently, irrigation farming, launching of villagization, climate variability as in series of droughts, construction of water dam and the rapid expansion of Prosopis juliflora were the major drivers of LULC changes in the study area. Although we found some positive developments such as improvement on infrastructural and social services (e.g. school and domestic water supply), income diversification and ecological benefits from Prosopis juliflora (e.g. saline soil treatment, carbon sequestration and soil erosion control), there were a range of negative impacts resulting from LULC changes in the study area. LULC changes reduced quality of rangeland resources as the ecologically and economically valuable indigenous tree and grass varieties were significantly degraded. As a result, the traditional pastoral livelihood system has been much vulnerable with the LULC dynamism of the study area. Furthermore, the implementation of the villagization programme has brought socioeconomic impacts on the community and challenges on the ecology, e.g. changing productive rangeland to irrigation crop farms. Our research results, thus, suggest the urgent need for relevant policy interventions in support of the pastoral livelihoods and landscapes with the modification in the implementation of villagization as well as irrigation farming programmes and its better management and controlling Prosopis juliflora expansion in the study area.

Cleaner approach for the cascade production of nanocellulose, nanohemicellulose and nanolignin from Prosopis juliflora.

This research focuses on developing a cleaner approach for the cascade production of nanocellulose, nanohemicellulose and nanolignin from Prosopis juliflora biomass. Via screening experiments, LA2/ChCl NADES was selected for selective hemicellulose solubilization in the first stage, and FA3/ChCl was selected for lignin solubilization in the second stage. This two-stage cascade process integrated with microwave gives a higher recovery yield (96.8 % cellulose, 92.43 % hemicellulose and 90.56 % lignin). Subsequently, recovered particles were converted into nanoparticles using intense ultrasonication. The produced nanocellulose, nanohemicellulose and nanolignin typically had a spherical structure with an average particle size of 87.4 ± 5.1 nm, 68.8 ± 2.1 nm, and 77.8 ± 2.6 nm. Nanoparticles produced by this study have many potential applications, especially in food, biomedical and packaging sectors. In addition, 80 % of LA2/ChCl NADES and 98 % of ethanol were recovered and reused. This approach of the holistic utilization of an invasive species rather than eliminating it paves a path towards environmental sustainability.

Study on the Impact and Water Absorption Performance of Prosopis juliflora & Glass Fibre Reinforced Epoxy Composite Laminates.

Current global trends demand the replacement of synthetic fibres with natural fibres in polymeric composites. The present work makes use of Prosopis juliflora, a plant that is a threat to the environment as a partial replacement in a hybrid composite. Individual Prosopis juliflora fibres are added to matrices at ratios of 12, 6, 9 and 8 wt % and glass fibres are added discretely at ratios of 28, 24, 21 and 32 wt % into matrices as well. The composites are prepared with four different combinations and tested in terms of the mechanical benefits and water absorption performance. This work exploits the mechanical advantage of impact energy in addition to producing Prosopis juliflora particles, fibre glass mats, and resin appropriate for structural uses. Water absorption tests are conducted for four different compositions. Among the four samples, sample 3 (9 wt % Prosopis juliflora fibres and 21 wt % glass fibres) has a higher rate of water absorption than the others, although sample 2 (6 wt % Prosopis juliflora fibres and 24 wt % glass fibres) has a lower rate. The difference in the quantity of water absorption between the hybrid composites can be attributed to the weight percentage of fibres. On the other hand, sample 1 (12 wt % Prosopis juliflora fibres and 28 wt % glass fibres) is reported to have absorbed 2.6 J of energy in the impact strength test. The increase in impact strength is attributed to the increase in the weight percentage of glass fibres. A scanning electron microscope is employed to study the fractured surfaces of the composites. This study shows that the developed hybrid composite could be employed in structural and automotive applications because of its improved impact strength and water resistance.

Effect of Prosopis Juliflora Thorns on Mechanical Properties of Plastic Waste Reinforced Epoxy Composites.

Plastics are unavoidable at this times, putting our planet in danger. The Prosopis juliflora (PJ) thorns are collected, processed, and powdered. The mechanical characteristics of these powders are examined when combined with polymer composites. Pores are the main cause of moisture input, hence using powder filler materials reduces the number of pores in the composite, increasing water resistance. The composites are made by altering three parameters: waste plastic content, filler powder composition, and chemical treatment. It was discovered that the integration of thorn powder increased the wear resistance. The composites were tested in accordance with ASTM standards, and the results were optimized. Based on the results, composite specimens were created and tested for validation.

Effects of treating Prosopis juliflora pods with multienzyme, with and without bacterial cultures on in vitro dry matter digestibility (IVDMD), fermentation kinetics, and performance of growing pigs.

This study was conducted to determine the effects of treating Prosopis juliflora pods with multienzyme and bacterial cultures on in vitro dry matter digestibility (IVDMD), fermentation kinetics, and performance of growing pigs. Experiment one consisted of a pepsin-pancreatine hydrolysis method to simulate, in vitro, the pig digestive system and was followed by in vitro gas production to assess fermentation kinetics. Samples of ground Prosopis pod meal (GPPM) were allocated to four treatments with three replicates each. Treatments included GPPM treated with multienzyme (Natuzyme®) (T1); untreated (GPPM) (T2); GPPM fermented with (Lactobacillus plantarum MTD1 Ecosyl ®) (T3), and GPPM treated using natural fermentation (T4). The second experiment assessed the performance of pigs fed the best treatment from experiment 1. Thirty Landrace × large white crosses of 20 ± 2 kg were allotted to five treatments with six pigs each (replicates). The dietary treatments were PC, 0% GPPM + enzyme; NC, 0% GPPM and 0% enzyme; D1, 10% GPPM + enzyme; D2, 20% GPPM + enzyme; and D3, 30% GPPM + enzyme. A randomized complete block design was used for both experiments. Enzyme treatment (T1) and T3 improved the IVDMD of the GPPM compared to T2 by 3.68% and 1.2%, respectively (p < 0.05). Cumulative gas was highest and Tmax lowest for T1 but significantly different only to T4 (p < 0.05). Average daily gain and intake were highest for pigs fed GPPM up to 10% (PC, D1). Feed conversion ratio increased with the level of GPPM in the diet. The results suggest Prosopis juliflora pods treated with enzymes can be added in pig diets up to 30%.

Adsorptive decontamination of doxycycline hydrochloride via Prosopis juliflora activated carbon: Parameter optimization and disposal study.

This study deals with the removal of doxycycline hydrochloride (DOX) antibiotic, from aqueous environment by using Prosopis juliflora activated carbon (PJAC). PJAC was synthesized by chemical activation and pyrolysis of Prosopis juliflora. It was characterized by employing Fourier transform infrared spectroscopy (FTIR), scanning electron microscope-energy dispersive X-ray analysis (SEM-EDX), X-ray diffraction analysis (XRD), and Brunauer-Emmett-Teller (BET) techniques. The specific surface area, pore volume, and pore diameter were evaluated as 320.45 m2 /g, 0.176 cm3 /g, and 2.65 nm, respectively. Different functional groups (O-H, C-O, C=C, C-N, and C-C) present on PJAC promoted the adsorption of DOX. The influence of various adsorption parameters suggested by central composite design (CCD) model was determined using response surface methodology (RSM), and interactive effects of these were optimized. The thermodynamic and kinetic studies performed at optimized conditions, exhibited that adsorption was spontaneous and endothermic. The experimental data were well described with Langmuir, Redlich-Peterson, and Freundlich isotherm models while kinetics data were well described by pseudo second order. The excellent interactions between the PJAC and DOX resulted maximum adsorption capacity as 57.11 mg/g. The adsorption mechanisms was dominated by π - π interactions and hydrogen bonding. Moreover, almost complete encapsulation of DOX was achieved by stabilization of exhausted PJAC. PRACTITIONER POINTS: A wild harmful plant Prosopis juliflora was used to synthesize a low-cost and eco-friendly bio-sorbent PJAC. Adsorptive ability of PJAC was quantified for adsorption of DOX antibiotic from its aqueous solution. DOX uptake on PJAC was mainly governed by л-л EDA interactions and hydrogen bonding.

Ecological Networks in Urban Forest Fragments Reveal Species Associations between Native and Invasive Plant Communities.

Forest fragments are characteristic features of many megacities that have survived the urbanisation process and are often represented by unique assemblages of flora and fauna. Such woodlands are representations of nature in the city-often dominated by non-native and invasive species that coexist with resilient native congeners and purposefully introduced flora. These forest fragments also provide significant ecosystem services to urban society and therefore, understanding their compositional patterns is of considerable importance for conservation and management. In this work, we use a complex network approach to investigate species assemblages across six distinct urban forest fragments in the South Delhi Ridge area of the National Capital Territory, India. We generate bipartite ecological networks using conventional vegetation sampling datasets, followed by network partitioning to identify multiple cliques across the six forest fragments. Our results show that urban woodlands primarily form invasive-native associations, and that major invasive species, such as Prosopis juliflora and Lantana camara exclude each other while forming cliques. Our findings have implications for the conservation of these urban forests and highlight the importance of using network approaches in vegetation analysis.

Identification of a vicilin-like major allergen from Prosopis juliflora exhibiting cross- reactivity with legume food allergens.

BACKGROUND: Prosopis juliflora is a clinically relevant allergic sensitizer worldwide and shares cross-reactivity with allergens from several tree pollen and food. The present study aims to purify and immunobiochemically characterize a major allergen from Prosopis pollen. The allergen was further investigated for its cross-reactivity with legume allergens. METHODS: Prosopis extract was fractionated by Q Sepharose and Superdex 75 gel filtration column to purify the allergen. Specific IgE against purified protein was estimated via ELISA and immunoblot. The protein was subjected to mass spectrometric analysis. Glycan characterization was performed by Schiff staining and lectin binding assay followed by deglycosylation studies. The functional activity of the purified protein was evaluated by the basophil activation test. Cross-reactivity was assessed by inhibition studies with legume extracts. RESULTS: A 35 kDa protein was purified and showed 75% IgE reactivity with the patients' sera by ELISA and immunoblot. Glycan characterization of protein demonstrated the presence of terminal glucose and mannose residues. A reduction of 40% and 27% in IgE binding was observed upon chemical and enzymatic deglycosylation of the protein, respectively. The glycoprotein allergen upregulates the expression of CD203c on basophils which was significantly reduced upon deglycosylation, signifying its biological ability to activate the effector cells. The identified protein shared significant homology with Lup an 1 from the lupine bean. Immunoblot inhibition studies of the purified allergen with legume extracts underlined high cross-reactive potential. Complete inhibition was observed with peanut and common bean, while up to 70% inhibition was demonstrated with soy, black gram, chickpea, and lima bean. CONCLUSION: A 35 kDa vicilin-like major allergen was isolated from P. juliflora. The protein possesses glycan moieties crucial for IgE binding and basophil activation. Furthermore, the purified protein shows homology with Lup an 1 and exhibits cross-reactivity with common edible legume proteins.